Analysis of Turnip Crinkle Virus Effects on the Innate and Adaptive Immunity in Arabidopsis Thaliana
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چکیده
..............................................................................................119 Introduction...........................................................................................120 Materials and Methods..............................................................................121 Results.................................................................................................125 Discussion............................................................................................134 References.............................................................................................140 Figure Legends........................................................................................144 Figures..................................................................................................148 Tables..................................................................................................156 CHAPTER 4: Evaluation of silencing pathway gene expression and miRNA accumulation in Turnip crinkle virus infections of Arabidopsis thaliana......159 Abstract...............................................................................................160 Introduction...........................................................................................161 Materials and Methods..............................................................................164 Results and Discussion.............................................................................167 Conclusions...........................................................................................176 References.............................................................................................179
منابع مشابه
Genome-wide transcriptomic analysis reveals correlation between higher WRKY61 expression and reduced symptom severity in Turnip crinkle virus infected Arabidopsis thaliana
Turnip crinkle virus (TCV) is a carmovirus that infects many Arabidopsis ecotypes. Most studies mainly focused on discovery of resistance genes against TCV infection, and there is no Next Generation Sequencing based comparative genome wide transcriptome analysis reported. In this study, RNA-seq based transcriptome analysis revealed that 238 (155 up-regulated and 83 down-regulated) significant d...
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Comparison of the symptoms caused by turnip crinkle virus strain M (TCV-M) and TCV-B infection of a resistant Arabidopsis thaliana line termed Di-17 demonstrates that TCV-B has a greater ability to spread in planta. This ability is due to a single amino acid change in the viral movement protein p8 and inversely correlates with p8 RNA binding affinity.
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The 3' untranslated region (UTR) of turnip crinkle virus (TCV) RNA is 253 nt long (nt 3798-4050) with a 27 nt hairpin structure near its 3' terminus. In this study, the roles of the 3' UTR in virus accumulation were investigated in protoplasts of Hibiscus cannabinus L. and Arabidopsis thaliana (L.) Heynh. Our results showed that, in Hibiscus protoplasts, the minimal 3' UTR essential for TCV acc...
متن کاملCRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity.
Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the ATPase and S5 domains characteristic of Microchidia (MORC) proteins; these proteins are associated with DNA modification and repair. Here we show that CRT1 and its closest homologue, CRH1...
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Plant hormones play pivotal roles as signals of plant-pathogen interactions. Here, we report that exogenous application of salicylic acid (SA), jasmonic acid (JA), ethephon (ETH), and abscisic acid (ABA) can reduce Turnip crinkle virus (TCV) accumulation in systemic leaves of Arabidopsis thaliana during early infection. SA and ABA are more efficient and confer a longer-lasting resistance agains...
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